Automatic calibration of retention times and response factors are automatically replaced with the calibrators data. Run the Standard. Program the sequence table to run the #68 FAME standard in first available position of the ALS, the NIH reference – F control in the second position, and a hexane solvent blank in the third position.Centrifuge the sample at 1600 rpm for 5 minutes.Incubate the sample at room temperature for 5 minutes.Add 50 µl of sodium methoxide to the sample in hexane.If the sample is in chloroform, evaporate the solvent with nitrogen and add 1 ml of hexane. If the sample is in powder form, dilute directly with 1 ml of hexane. Transfer 5 mg of sample into a test tube.Keep the reference standard refrigerated at 2-8☌.Dilute to a final concentration of 20 mg/ml with additional hexane.Determine concentration by gravimetric analysis. Transfer the contents of 1 vial of #68A STANDARD to a small bottle with a cap.Reference Standard #68A (NU-CHEK-PREP, INC.).J & W Fused Silica Capillary Column DB-225 30 M X 0.2 mm, 0.33 µ film.The FAMEs are identified and quantitated using capillary gas chromatography with flame ionization detection (FID). This hydrolysis and methylation occur at room temperature when reacted with 1N sodium methoxide. These fatty acids undergo methylation of the carboxylic acid function to form fatty acid methyl esters (FAME). Phospholipids are hydrolyzed to free their fatty acids from the sn-1 and sn-2 glycerol backbone. Miscibility of Phospholipid Binary Mixtures.Phase Transition Temperatures for Glycerophospholipids.Fatty Acid Analysis of Phospholipids by GC/FID.Detection Methods for Thin Layer Chromatography.Thin Layer Chromatography (TLC) Analysis of Phospholipids.Determination of Phospholipid Oxidation by UV/VIS Spectroscopy.Procedure for Preparation of Cationic Lipid/Oligomer Complex.Preparation of Cationic Liposomes & Transfection of Cells.Current Applications of Liposome Formulations.Preparing Large, Unilamellar Vesicles by Extrusion (LUVET). Preparation of Multilamellar Liposomes (LMV).
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